![I'm looking to do fluorescence quantitation using your Quantum MESF kits. One concern I had however was that my unlabeled cells have a lower fluorescence intensity than the blank bead. Why is I'm looking to do fluorescence quantitation using your Quantum MESF kits. One concern I had however was that my unlabeled cells have a lower fluorescence intensity than the blank bead. Why is](https://www.bangslabs.com/sites/default/files/imce/images/autofluorescence.jpg)
I'm looking to do fluorescence quantitation using your Quantum MESF kits. One concern I had however was that my unlabeled cells have a lower fluorescence intensity than the blank bead. Why is
![FL-1 Histograms of MESF beads mixtures (Quantum ™ 24 premixed FITC MESF... | Download Scientific Diagram FL-1 Histograms of MESF beads mixtures (Quantum ™ 24 premixed FITC MESF... | Download Scientific Diagram](https://www.researchgate.net/profile/Veena-Kapoor/publication/252020781/figure/fig1/AS:669527587176466@1536639224965/FL-1-Histograms-of-MESF-beads-mixtures-Quantum-24-premixed-FITC-MESF-beads-Region-M1.png)
FL-1 Histograms of MESF beads mixtures (Quantum ™ 24 premixed FITC MESF... | Download Scientific Diagram
Considerations in MESF-bead based assignment of absolute fluorescence values to nanoparticles and extracellular vesicles by flow
![Fluorescence calibration. (A) Representative flow cytometry dot plots... | Download Scientific Diagram Fluorescence calibration. (A) Representative flow cytometry dot plots... | Download Scientific Diagram](https://www.researchgate.net/publication/358427991/figure/fig2/AS:1121417546465280@1644378186560/Fluorescence-calibration-A-Representative-flow-cytometry-dot-plots-of-Quantum-Alexa.png)
Fluorescence calibration. (A) Representative flow cytometry dot plots... | Download Scientific Diagram
![Establishing receptor quantitation and the FACS gating strategy. (a)... | Download Scientific Diagram Establishing receptor quantitation and the FACS gating strategy. (a)... | Download Scientific Diagram](https://www.researchgate.net/publication/255987101/figure/fig2/AS:214276892565505@1428099000759/Establishing-receptor-quantitation-and-the-FACS-gating-strategy-a-Gating-and-quality.png)
Establishing receptor quantitation and the FACS gating strategy. (a)... | Download Scientific Diagram
![Validation of cell‐based fluorescence assays: Practice guidelines from the ICSH and ICCS – part III – analytical issues - Tangri - 2013 - Cytometry Part B: Clinical Cytometry - Wiley Online Library Validation of cell‐based fluorescence assays: Practice guidelines from the ICSH and ICCS – part III – analytical issues - Tangri - 2013 - Cytometry Part B: Clinical Cytometry - Wiley Online Library](https://onlinelibrary.wiley.com/cms/asset/845c65d9-bda6-4723-90db-942dbd02c6d1/cytob21106-fig-0002-m.jpg)
Validation of cell‐based fluorescence assays: Practice guidelines from the ICSH and ICCS – part III – analytical issues - Tangri - 2013 - Cytometry Part B: Clinical Cytometry - Wiley Online Library
![Considerations for MESF-bead based assignment of absolute fluorescence values to nanoparticles and extracellular vesicles by flow cytometry | bioRxiv Considerations for MESF-bead based assignment of absolute fluorescence values to nanoparticles and extracellular vesicles by flow cytometry | bioRxiv](https://www.biorxiv.org/content/biorxiv/early/2021/03/01/2021.03.01.433358/F3.large.jpg)